Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: ETV7 was highly expressed in CRC. A CRC tissues ( n = 20) and paracancerous ( n = 20) tissues were subjected to immunochemistry staining of ETV7. B ETV7 expression in CRC ( n = 275) and control samples ( n = 349) based on the information from TCGA database. C Methylation level of the ETV7 promoter in CRC ( n = 313) and control samples ( n = 37) based on the information from TCGA database. D The protein expression of ETV7 was checked in two types of CRC cells (RKO and HCT116) and normal colorectal cells (NCM460)

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Staining, Expressing, Control, Methylation

Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: Correlation of ETV7 expression and clinical features in patients with CRC

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Expressing

Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: ETV7 promoted the amplification and migration of CRC cells. A The protein expression of ETV7 was checked in RKO cells transfected with pcDNA empty vector (vector) or pcDNA-ETV7 (ETV7). B Cell viability was measured in RKO cells transfected with pcDNA empty vector (vector) or pcDNA-ETV7 (ETV7). n = 3. C, D Cell colony formation and migration ability were examined in RKO cells transfected with pcDNA empty vector (vector) or pcDNA-ETV7 (ETV7). n = 3. E The protein expression of ETV7 was checked in siCtrl, siETV7#1, and siETV7#2 HCT116 cells. F Cell viability was measured in siCtrl, siETV7#1, and siETV7#2 HCT116 cells. n = 3. G, H Cell colony formation and migration ability were examined n siCtrl, siETV7#1, and siETV7#2 HCT116 cells. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Amplification, Migration, Expressing, Transfection, Plasmid Preparation

Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: ETV7 reduced apoptosis and cell cycle arrest in colorectal cancer cells. A Rate of apoptosis in RKO cells transfected with pcDNA empty vector (vector) or pcDNA-ETV7 (ETV7). n = 3. B Rate of apoptosis in siCtrl, siETV7#1, and siETV7#2 HCT116 cells. n = 3. C Cell cycle distribution of RKO cells transfected with pcDNA empty vector (vector) or pcDNA-ETV7 (ETV7). n = 3. D Cell cycle distribution of siCtrl, siETV7#1, and siETV7#2 HCT116 cells. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Transfection, Plasmid Preparation

Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: ETV7 positively regulated the expression of IFIT3. A–C The qPCR ( n = 3) and WB analyses detected IFIT3 expression in RKO cells transfected with pcDNA-ETV7 and HCT16 cells transfected with siETV7. D, E IFIT3 promoter luciferase activity was detected in RKO and HCT16 cells 24 h following transfection with pcDNA-ETV7 or siRNAs targeting ETV7. n = 3. F Pearson’s correlation of ETV7 and IFIT3 expression using data from TCGA database. *** p < 0.001

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Expressing, Transfection, Luciferase, Activity Assay

Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: IFIT3 knockdown inhibited the cellular function in ETV7-overexpressing cells. A The protein expression of IFIT3 in RKO cells transfected with pcDNA-ETV7 and pcDNA-ETV7 + siIFIT3. B Viability of RKO cells transfected with pcDNA-ETV7 and pcDNA-ETV7 + siIFIT3. n = 3. C Colony numbers of RKO cells transfected with pcDNA-ETV7 and pcDNA-ETV7 + siIFIT3. n = 3. D Numbers of migrated RKO cells transfected with pcDNA-ETV7 and pcDNA-ETV7 + siIFIT3. n = 3. ** p < 0.01; *** p < 0.001

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Knockdown, Cell Function Assay, Expressing, Transfection

Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: IFIT3 overexpression rescued the cellular function in ETV7-silenced colorectal cancer cells. A IFIT3 expression in HCT116 cells transfected with siETV7 and siETV7 + pcDNA-IFIT3 was detected using WB. B Viability of HCT116 cells transfected with siETV7 and siETV7 + pcDNA-IFIT3. n = 3. C, D Colony numbers and numbers of migrated HCT116 cells transfected with siETV7 and siETV7 + pcDNA-IFIT3. n = 3. ** p < 0.01; *** p < 0.001

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Over Expression, Cell Function Assay, Expressing, Transfection

Journal: Functional & Integrative Genomics

Article Title: ETV7 promotes colorectal cancer progression through upregulation of IFIT3

doi: 10.1007/s10142-023-01282-y

Figure Lengend Snippet: The expression of ETV7 in normal tissue and CRC tissue by IHC

Article Snippet: Of note, elevated expression of ETV7 has been linked to B lymphomagenesis (Cardone et al. ), hematopoietic malignancy (Carella et al. ; Kawagoe et al. ), hepatocellular carcinoma (Matos et al. ), and bladder cancer (Li et al. ).

Techniques: Expressing